AAV-SaCas9在体敲除技术服务

 

HANHENGSHENGWUJISHUGONGCHENGSHIYUNYONGCRISPR/Cas9JIYINQIAOCHUJISHU，JIANGXIANXIANGGUANBINGDUYUSaCas9JIEHE，JIAAV-SaCas9，KEYISHIXIANGAOXIAOZAITIJIYINQIAOCHU，YESHIZUIXINZUILINGXIANDEZAITIJIYINQIAOCHUJISHU。 YULEJIEAAVBUTONGDEXUEQINGXINGJIDIDUQINGZIXUNQUANGUOMIANFEIFUWUREXIAN：400-092-0065

 

 

AAV其他相关特色服务

 

1.    腺相关病毒AAV包装服务

2.    AAV在体自噬流检测服务

3.    AAV光遗传技术服务

 

 

AAV-SaCas9简介

 

ZUIZAOZAIXIJUNZHONGBEIFAXIAN，CRISPR-Cas9XITONGNENGGOUQIEGEDNA，CHONGDANGLEDUIKANGBINGDUGANRANDEYIGEZHONGYAOFANGYUJIZHI。JINGUANXUDUODEWEISHENGWUWUZHONGDOUYONGYOUZHEIYIXITONG，YANJIURENYUANYOUXIANXUANZEGAIZAOLELAIZIHUANONGLIANQIUJUNDECas9MEI(SpCas9)LAIGAIBIANGAODENGSHENGWUDEDNA，BINGYICHENGWEIYIXILIEGAODUTONGYONGDEJIYINZUXIUSHIJISHUDEJICHU。 

 

YAOXIANGRAOLUANCHENGTIDONGWUZHONGDEJIYIN，BIXUYAOLIYONGYIXIEZAITIJIANGCRISPR-Cas9XITONGDEGUANJIANYUANJIANDAORUDAOXIBAOZHONG。YOUYUBUHUIYINQIRENLEIJIBINGBINGYIZAIOUZHOUHUODELINCHUANGJIANGUANJIGOUDEPIZHUN，XIANXIANGGUANBINGDU(AAV)BEISHIWEISHIZUIYOUQIANJINGDEHOUXUANZAITIZHIYI。RANER，AAVWEIXIAODEFUZAINENGLISHIDETONGSHIBAOZHUANGSpCas9MEIHEQITAJIYINBIANJIBIXUDEYUANJIANJINRUDAODANGEBINGDUKELIZHONGCHENGWEIYIGETIAOZHAN。 

 

MASHENGLIGONGDEKEXUEJIAZHANGFENGYANJIUYUANZUIXINDEYANJIUFAXIANYIZHONGLAIZIJINHUANGSEPUTAOQIUJUNDECas9HESUANMEI(SaCas9)，TABISpCas9XIAO25%，ZAIAAVBINGDUDEBAOZHUANGRONGLIANGFANWEIZHINEI，CONGERWEIAAVDEBAOZHUANGWENTITIGONGLEYIGEJIEJUEFANGAN。

 

 

 2015 Apr 9;520(7546):186-91

 

 

汉恒生物技术工程师运用CRISPR/Cas9基因敲除技术，将腺相关病毒与SaCas9结合，即AAV-SaCas9，可以实现高效在体基因敲除，也是最新最领先的在体基因敲除技术。并且可以根据动物靶器官，选择不同血清型的AAV（不同血清型AAV对各组织器官的亲和性见下表），是目前最有效的在体基因敲除技术。

 

 

 

 

AAV载体不同血清型

 

YANJIUFAXIAN AAV JUYOUDUOZHONGXUEQINGXING，GEZHONGBUTONGXUEQINGXINGDE AAV ZAITIDEZHUYAOQUBIESHIYIQIAODANBAIBUTONG，YINCIDUIBUTONGDEZUZHIHEXIBAODEZHUANRANXIAOLVCUNZAICHAYI。MUQIANHANHENGSHENGWUZAIBAOZHUANGXIANXIANGGUANBINGDUSHIYOU12 ZHONGBUTONGDE AAV XUEQINGXINGKEGONGKEHUXUANZE，JIANYIKEHUZHENDUIBUTONGZUZHIQIGUANXUANZEXIANGYINGXUEQINGXINGDE AAV BINGDU，JIANBIAO 1。

 

BIAO1.12ZHONGBUTONGXUEQINGXINGAAVDUIGEZUZHIQIGUANXIBAODEQINHEXING

 

  

 

 

AAV-SaCas9服务流程

 

  

 

 

 

参考文献

 

1、Laterodorsal tegmentum interneuron subtypes oppositely regulate olfactory cue-induced innate fear；

 （2016年发表于Nature Neuroscience，作者：段树民）

 

2、LRP6 acts as a scaffold protein in cardiac gap junction assembly；

 （2016年发表于Nature Communications，作者：陈义汉）

 

3、Characterization of fowl adenovirus serotype 4 circulating in chickens in China；

 （2019年发表于Vet microbiology，作者：fanghua wei）

 

4、Long noncoding RNA HIF1A-AS2 facilitates cell survival and migration by sponging miR-33b-5p to modulate SIRT6 expression in osteosarcoma ；

 （2019年发表于Biochem Cell Biology，作者：赵政旭）

 

5、Expression levels of microRNAs that are potential cytochrome P450 regulators in cynomolgus macaques；

，作者：Uno Y）