在体敲除AAV-saCas9

 

AAV-SaCas9在体敲除技术服务

 

HANHENGSHENGWUJISHUGONGCHENGSHIYUNYONGCRISPR/Cas9JIYINQIAOCHUJISHU,JIANGXIANXIANGGUANBINGDUYUSaCas9JIEHE,JIAAV-SaCas9,KEYISHIXIANGAOXIAOZAITIJIYINQIAOCHU,YESHIZUIXINZUILINGXIANDEZAITIJIYINQIAOCHUJISHU。 YULEJIEAAVBUTONGDEXUEQINGXINGJIDIDUQINGZIXUNQUANGUOMIANFEIFUWUREXIAN:400-092-0065

 

 

AAV其他相关特色服务

 

1.    腺相关病毒AAV包装服务

2.    AAV在体自噬流检测服务

3.    AAV光遗传技术服务

 

 

AAV-SaCas9简介

 

ZUIZAOZAIXIJUNZHONGBEIFAXIAN,CRISPR-Cas9XITONGNENGGOUQIEGEDNA,CHONGDANGLEDUIKANGBINGDUGANRANDEYIGEZHONGYAOFANGYUJIZHI。JINGUANXUDUODEWEISHENGWUWUZHONGDOUYONGYOUZHEIYIXITONG,YANJIURENYUANYOUXIANXUANZEGAIZAOLELAIZIHUANONGLIANQIUJUNDECas9MEI(SpCas9)LAIGAIBIANGAODENGSHENGWUDEDNA,BINGYICHENGWEIYIXILIEGAODUTONGYONGDEJIYINZUXIUSHIJISHUDEJICHU。 

 

YAOXIANGRAOLUANCHENGTIDONGWUZHONGDEJIYIN,BIXUYAOLIYONGYIXIEZAITIJIANGCRISPR-Cas9XITONGDEGUANJIANYUANJIANDAORUDAOXIBAOZHONG。YOUYUBUHUIYINQIRENLEIJIBINGBINGYIZAIOUZHOUHUODELINCHUANGJIANGUANJIGOUDEPIZHUN,XIANXIANGGUANBINGDU(AAV)BEISHIWEISHIZUIYOUQIANJINGDEHOUXUANZAITIZHIYI。RANER,AAVWEIXIAODEFUZAINENGLISHIDETONGSHIBAOZHUANGSpCas9MEIHEQITAJIYINBIANJIBIXUDEYUANJIANJINRUDAODANGEBINGDUKELIZHONGCHENGWEIYIGETIAOZHAN。 

 

MASHENGLIGONGDEKEXUEJIAZHANGFENGYANJIUYUANZUIXINDEYANJIUFAXIANYIZHONGLAIZIJINHUANGSEPUTAOQIUJUNDECas9HESUANMEI(SaCas9),TABISpCas9XIAO25%,ZAIAAVBINGDUDEBAOZHUANGRONGLIANGFANWEIZHINEI,CONGERWEIAAVDEBAOZHUANGWENTITIGONGLEYIGEJIEJUEFANGAN。

 

 

 2015 Apr 9;520(7546):186-91

 

 

汉恒生物技术工程师运用CRISPR/Cas9基因敲除技术,将腺相关病毒与SaCas9结合,即AAV-SaCas9,可以实现高效在体基因敲除,也是最新最领先的在体基因敲除技术。并且可以根据动物靶器官,选择不同血清型的AAV(不同血清型AAV对各组织器官的亲和性见下表),是目前最有效的在体基因敲除技术。

 

 

 

 

AAV载体不同血清型

 

YANJIUFAXIAN AAV JUYOUDUOZHONGXUEQINGXING,GEZHONGBUTONGXUEQINGXINGDE AAV ZAITIDEZHUYAOQUBIESHIYIQIAODANBAIBUTONG,YINCIDUIBUTONGDEZUZHIHEXIBAODEZHUANRANXIAOLVCUNZAICHAYI。MUQIANHANHENGSHENGWUZAIBAOZHUANGXIANXIANGGUANBINGDUSHIYOU12 ZHONGBUTONGDE AAV XUEQINGXINGKEGONGKEHUXUANZE,JIANYIKEHUZHENDUIBUTONGZUZHIQIGUANXUANZEXIANGYINGXUEQINGXINGDE AAV BINGDU,JIANBIAO 1。

 

BIAO1.12ZHONGBUTONGXUEQINGXINGAAVDUIGEZUZHIQIGUANXIBAODEQINHEXING

 

  

 

 

AAV-SaCas9服务流程

 

  

 

 

 

参考文献

 

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 (2016年发表于Nature Neuroscience,作者:段树民)
 
2、LRP6 acts as a scaffold protein in cardiac gap junction assembly;
 (2016年发表于Nature Communications,作者:陈义汉)
 
3、Characterization of fowl adenovirus serotype 4 circulating in chickens in China;
 (2019年发表于Vet microbiology,作者:fanghua wei)
 
4、Long noncoding RNA HIF1A-AS2 facilitates cell survival and migration by sponging miR-33b-5p to modulate SIRT6 expression in osteosarcoma ;
 (2019年发表于Biochem Cell Biology,作者:赵政旭)
 
5、Expression levels of microRNAs that are potential cytochrome P450 regulators in cynomolgus macaques;
,作者:Uno Y)
 
 
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