QPCR检测

 

qPCR背景介绍

 

ZAISHISHIYINGGUANGDINGLIANGPCRZHONG,DUIMEIYILUNXUNHUANDEYINGGUANGXINHAOQIANGDUJINXINGJIANCEBINGJILU,SUIZHEFANYINGDEJINXING,JIANCEDAODEYINGGUANGXINHAOKEYIHUIZHICHENGYITIAOQUXIAN,JIWEIKUOZENGQUXIAN。YIBANERYAN,KUOZENGQUXIANKEYIFENCHENGSANGEJIEDUAN:JIXIANQI(Baseline phase),ZHISHUZENGZHANGQI(Log-linear phase)HEPINGTAIQI(Plateau phase)(TUYI)。ZAIKUOZENGZAOQIJIJIXIANQI,KUOZENGDEYINGGUANGXINHAOBEIYINGGUANGBEIJINGXINHAOSUOYANGAI,WUFAPANDUANCHANWULIANGDEBIANHUA。ZAIPINGTAIQI,KUOZENGCHANWUYIBUZAICHENGZHISHUJIDEZENGJIA,PCRDEZHONGCHANWULIANGYUQISHIMOBANLIANGZHIJIANWUXIANXINGGUANXI,SUOYIGENJUZUIZHONGDEPCRCHANWULIANGBUNENGJISUANCHUCHUSHIMOBANLIANG。ZHIYOUZAIYINGGUANGXINHAOZHISHUKUOZENGJIEDUAN,PCRCHANWULIANGDEDUISHUZHIYUQISHIMOBANLIANGZHIJIANCUNZAIXIANXINGGUANXI,KEZAICIJIEDUANJINXINGDINGLIANGFENXI。

 

 

流程图

 

 

 

汉恒提供服务项目

 

HANHENGSHENGWUKEJI(SHANGHAI)YOUXIANYABOappGUANWANGTIGONGCONGYINWUSHEJI,XIBAOHUOZHEZUZHIYANGPINCHULI,qPCRJIANCEYIJIWANZHENGDESHUJUBAOGAODENGFUWU。WEININTIGONGZUIYOUZHIDEFUWU,WEININDEKEYANBAOJIAHUHANG。

KEHUTIGONG:

1.细胞:细胞数106-107;组织:总量>100mg;DNA或RNA样品:总量>2 μg

2.BAJIYINXINXI:BAOKUOJIYINXULIEHUOGenBank DENGLUHAODENG

3.BEIJINGZILIAO:BAOKUOSHENGWUWUZHONGXINXI、JIYINGONGNENG、YONGTU、TEXING、DNA/RNA LAIYUANDENG

(BEIZHU:QINGZAISONGYANGQIAN,YUJISHUQUERENJUTISONGYANGQINGKUANG。)

 

 

实例展示:

 

(Hou Z ,Qin X ,Hu Y ,et al. 2019)

 

 

ZAIYANJIUZHANGQIDUANLIANBAOHUXINZANGDEFENZIJIZHIZHONG,YANJIURENYUANDUIYUNDONGDASHU(Exe-exo)HEJIUZUODASHU(Sed-exo)DEWAIMITIJINXINGmiRNA-seqHEqPCR,LAIQUEDINGYANJIUMUBIAOYUXINZANGBAOHUXIANGGUANDEmiR-342 5p。

 

 

CANKAOWENXIAN:

Hawkins, S. F. C, Guest, P. C. Multiplex Analyses Using Real-Time Quantitative PCR[J]. Methods in Molecular Biology,2016,1546:125-133.

Wong M L , Medrano J F . Real-time PCR for mRNA quantitation[J]. BioTechniques, 2005, 39(1):75-85

Hou Z , Qin X , Hu Y , et al. Longterm Exercise-Derived Exosomal miR-342-5p: A Novel Exerkine for Cardioprotection[J]. Circulation Research, 2019,124(9):1386-1400.

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