单萤(荧)光素酶检测试剂盒

 

实验原理

 

 

YINGHUOCHONGYINGGUANGSUMEI(firefly luciferase, fluc)NENGCUIHUAYINGGUANGSU(luciferin)CHANSHENGSHENGWUYINGGUANGFANYING,FANYINGGUOCHENGRUXIATUSUOSHI。

 


 

特点:

1.DIWUCUNZAISHI,CUIHUADIWUFACHUYINGGUANG

2.YINGGUANGLIANGDUHEYINGGUANGSUMEIDELIANGCHENGZHENGBI

 


 

GENJUYINGGUANGQIANGDU,FANYINGBIAODAZAITISHANGF-lucDEBIAODALIANG

 

 

应用

 

转录因子结合位点与启动子活性分析

 

ZHUANLUYINZI(Transcription factors,TF)SHIYIZHONGJUYOUTESHUJIEGOU、XINGSHIDIAOKONGJIYINBIAODAGONGNENGDEDANBAIZHIFENZI,YECHENGWEIFANSHIZUOYONGYINZI,ZHUANLUYINZIJINYUQIBAQIDONGZIZHONGDETEYISHUNXUJIEHE,CONGERZENGQIANGQIXIAYOUJIYINDEBIAODA。

YINGGUANGSUMEIBAOGAOJIYINSHIYANSHIJIANCEZHEILEIZHUANLUYINZIJIQIBAQIDONGZIZHONGTEYIXULIEJIEHEDEZHONGYAOSHOUDUAN

 


 

亚博app官网规格

 

 

 

实验步骤

 

YI、PEIZHISHIJI

1.1 Lysis Buffer:Lysis Buffer (5X) YONGSHUIXISHI5BEI,PEIZHICHENGGONGZUOYE;

1.2. Luciferase Reaction Reagent:YONGLuciferase Reaction Buffer(5 ml)RONGJIELuciferase Reaction SubstrateGANFEN,HUNYUNHOUFENZHUANG、BIGUANGBAOCUN,BIMIANFANFUDONGRONG。

 

ER、LIEJIEXIBAO

2.1 XIBAOLIEJIESHIMIDUYINGXIAOYU95%,XIBAOSHENGZHANGGUOMI,YIDAOZHILIEJIEBUCHONGFEN;ZHILISHUNZHUAN、BINGDUGANRANXIBAOHOU,XUPEIYANGYIDUANSHIJIAN(YIBAN48XIAOSHI),SHIYINGGUANGSUMEICHONGFENBIAODA;DIAOJIESHIYANCANSHU,YIMANZUSHANGSHUTIAOJIAN;

2.2 QUCHUPEIYANGJI,YONGPBSRUNXIYIBIAN,ZHUYIBUYAOJIANGXIBAOCHUIQI;JIARUSHILIANGDELysis Buffer。

2.3 JIANGXIBAOPEIYANGBANZHIYUYAOCHUANGSHANG,SHIWEN、QINGHUANGFUYU15FENZHONG;

2.4 JIANGLIEJIEWUZHUANYIDAOXINDEEPGUANZHONG,4oC、12,000rpmLIXIN1FENZHONG,QUSHANGQINGBEIYONG

 

SAN、YINGGUANGJIANCE

QU20μlLIEJIEWU,JIARU100μlPINGHENGZHISHIWENDELuciferase Reaction Reagent,HUNYUNHOUYUMEIBIAOYIZHONGJIANCEfLucXINHAO。

 

HANHENGYINGGUANGSUMEI(firefly)JIANCESHIJIHEJUYOUCAOZUOJIANDAN、JIANCEKUAIJIE、LINGMINDUGAODENGYOUDIAN,SHIKEYANGONGZUODEYOUZHIGONGJU!

 

 

 

 

Chinese, Simplified
 
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