Crispr/Cas9 腺病毒

 

Crispr/Cas9 简介

CRISPR (clustered, regularly interspaced, short palindromic repeats)SHIYIZHONGLAIZIXIJUNJIANGJIERUQINDEBINGDU DNA HUOQITAWAIYUAN DNA DEMIANYIJIZHI。MUQIAN,LAIZIStreptococcus pyogenes DECRISPR-Cas9XITONGYINGYONGZUIWEIGUANGFAN。Cas9 DANBAI(HANYOULIANGGEHESUANMEIJIEGOUYU,KEYIFENBIEQIEGEDNA LIANGTIAODANLIAN。Cas9SHOUXIANYUcrRNAJItracrRNAJIEHECHENGFUHEWU,RANHOUTONGGUOPAMXULIEJIEHEBINGQINRUDNA,XINGCHENGRNA-DNAFUHEJIEGOU,JINERDUIMUDEDNASHUANGLIANJINXINGQIEGE,SHIDNASHUANGLIANDUANLIE。

 

CRISPR-Cas9XITONGYIJINGCHENGGONGYINGYONGYUZHIWU、XIJUN、JIAOMU、YULEIJIBURUDONGWUXIBAO,SHIMUQIANZUIGAOXIAODEJIYINZUBIANJIXITONG。

 

TONGGUOJIYINGONGCHENGSHOUDUANDUIcrRNAHEtracrRNAJINXINGGAIZAO,JIANGQILIANJIEZAIYIQIDEDAOsgRNA(single guide RNA)。RONGHEDERNAJUYOUYUYESHENGXINGRNALEISIDEHUOLI,DANYINWEIJIEGOUDEDAOLEJIANHUAGENGFANGBIANYANJIUZHESHIYONG。TONGGUOJIANGBIAODAsgRNADEYUANJIANYUBIAODACas9DEYUANJIANXIANGLIANJIE,DEDAOKEYITONGSHIBIAODALIANGZHEDEZHILI,JIANGQIZHUANRANXIBAO,BIANNENGGOUDUIMUDEJIYINJINXINGCAOZUO。

 

汉恒生物创新性的首次将cas9和gRNA整合到腺病毒载体中,成功包装cas9-gRNA腺病毒,很大程度上弥补了crispr/cas9质粒感染效率低,从而导致敲除效率低的缺陷。

 

HANHENGSHENGWUTUICHUDECas9/gRNAXIANBINGDULIYONGcas9HEgRNAJIEHETEYIXING,TONGSHIJIEZHUXIANBINGDUGAOGANRANXIAOLV,GAOBIAODAXIAOLV,TIGAOcrispr/cas9JIYINQIAOCHUXIAOLV,SUODUANJIYINQIAOCHUZHOUQI,SHIDEJIYINQIAOCHUZHOUQIDUAN,CHENGBENDI,GENGYIYUCAOZUO。

 

 

Crispr/Cas9腺病毒特点:
   

1、KEGANRANDUOZHONGFENLIEQIHEFEIFENLIEQIXIBAO,GANRANXIAOLVGAO、QIAOCHUXIAOLVGAO;

2、CAOZUOJIANDAN,RONGYIZHANGWO;

3、JIYINQIAOCHUZHOUQIDUAN,CHENGBENDI;

4、SHIHEBURUDONGWUBUTONGZHONGSHUBUTONGJIYINDECAOZUO,SHIYONGFANWEIGUANG。

 

 

服务流程

 

 

 

 

 

参考文献

   1、Laterodorsal tegmentum interneuron subtypes oppositely regulate olfactory cue-induced innate fear;
     (2016年发表于Nature Neuroscience,作者:段树民)
   2、Endoplasmic reticulum stress induced by tunicamycin and thapsigargin protects against transient ischemic brain injury Involvement of PARK2-dependent mitophagy;
     (2016年发表于Autophagy作者:Xiangnan Zhang)
   3、SNX13 reduction mediates heart failure through degradative sorting of apoptosis repressor with caspase recruitment domain;
     (2016年发表于Nature Communications,作者:陈义汉)
   4、TRB3 links insulin IGF to tumour promotion by interacting with p62 and impeding autophagic proteasomal degradations;
     (2016年发表于Nature Communications,作者:胡卓伟)
   5、Suppression of KRas-mutant cancer through the combined inhibition of KRAS with PLK1 and ROCK;
     (2016年发表于Nature Communications,作者:刘明耀)
     (2019年发表于Nature Communications,作者:Zhang X)
   7、LRP6 acts as a scaffold protein in cardiac gap junction assembly;
     (2016年发表于Nature Communications,作者:陈义汉)
   8、Long-Range Chromosome Interactions Mediated by Cohesin Shape Circadian Gene Expression;
     (2016年发表于plos genetics,作者:Jun Yan )
   9、Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy;
     (2016年发表于Kidney International,作者:易凡)
   10、Roles of miR-1-1 and miR-181c in ventricular septal defects;
     (2016年发表于International Journal of Cardiology,作者:Li J)
   11、Intronic SMCHD1 variants in FSHD: testing the potential for CRISPR-Cas9 genome editing;
     (2019年发表于J Med Genet,作者:Balog J)
   12、Generation of a WWTR1 mutation induced pluripotent stem cell line, MUSIi012-A-1, using CRISPR/Cas9;
     (2019年发表于Stem Cell Research ,作者:Terbto P)
   13、Liposomal delivery of CRISPR/Cas9;
     (2019年发表于Cancer Gene Therapy,作者:Zhen S)
   14、Brain transcriptome profile after CRISPR-induced ghrelin mutations in zebrafish;
     (2019年发表于Fish Physiological and Biochemistry,作者: Soletto L)
   15、CRISPR/Cas9-mediated gene correction in hemophilia B patient-derived iPSCs;
     (2019年发表于Int J Hematol,作者: Seki R)
   16、A CRISPR/Cas9-based strategy to simultaneously inactivate the entire gene family in Candida orthopsilosis;
     (2019年发表于Future Microbiolgy,作者:Bottai D)
   17、Preparation of a new type 2 diabetic miniature pig model via the CRISPR/Cas9 system;
     (2019年发表于Cell Death&Disease,作者:Chen C)
   18、A CRISPR-Cas9 system for multiple genome editing and pathway assembly in Candida tropicalis;
     (2019年发表于Biotechnology and Bioengineering,作者:Shen W)
   19、MicroRNAs as Diagnostic, Prognostic, and Therapeutic Biomarkers in Prostate Cancer;
     (2019年发表于Crit Rev Eukaryot Gene Expr,作者:Amiri A)
   20、MicroRNA regulation of CTP synthase and cytoophidium in Drosophila melanogaster;
     (2019年发表于Experimental Cell Research,作者:Dzaki N)
   21、External validation of a panel of plasma microRNA biomarkers for lung cancer;
     (2019年发表于Biomakers in Medcine,作者:Li J)
   22、Kinetics Analysis of Circulating MicroRNAs Unveils Markers of Failed Myocardial Reperfusion ;
     (2019年发表于Clinical Chemistry,作者:Ali S)
   23、Cumulus cell-derived and maternal SIRT6 differentially regulates porcine oocyte meiotic maturation;
     (2019年发表于Theriogenology,作者:Yu T)
   24、Sirtuins and SIRT6 in Carcinogenesis and in Diet;
     (2019年发表于Internationl Journal of Molecular Science,作者:Souto EB)
   25、Transcription factor E2-2 inhibits the proliferation of endothelial progenitor cells by suppressing autophagy;
     (2016年发表于International Journal of Molecular Medicine,作者:Hong Wang)
   26、Effect of adenovirus-mediated RNA interference of IL-1β expression on spinal cord injury in rats;
     (2016年发表于Spinal Cord)
   27、miR-23b-3p regulates the chemoresistance of gastric cancer cells by targeting ATG12 and HMGB2;
     (2016年发表于Cell Death and Disease,作者: Q Zhao)
   28、Adipose-derived stem cells induce autophagic activation and inhibit catabolic response to pro-inflammatory cytokines in rat chondrocytes;
     (2016年发表于Osteoarthritis and Cartilage,作者: Jian Zhang)
   29、β-Arrestins promote podocyte injury by inhibition of autophagy in diabetic nephropathy;
     (2016年发表于 Cell Death and Disease,作者:F Yi )
   30、MicroRNA-30a downregulation contributes to chemoresistance of osteosarcoma cells through activating Beclin-1-mediated autophagy;
     (2016年发表于Oncology Reports,作者:Lifeng Lao)
   31、Luteolin alleviates post‐infarction cardiac dysfunction by up‐regulating autophagy through Mst1 inhibition;
     (2016年发表于J. Cell. Mol. Med.,作者:Dongdong Sun )
   32、Long non-coding RNA HNF1A-AS1 functioned as an oncogene and autophagy promoter in hepatocellular carcinoma through sponging hsa-miR-30b-5p;
     (2016年发表于Biochemical and Biophysical Research Communications,作者: Jiahong Dong)
   33、MST1 suppresses viability and promotes apoptosis of glioma cells via upregulating SIRT6 expression;
     (2019年发表于Journal of Integrative Neuroscience,作者:Zhu D)
   34、Long non-coding RNA HNF1A-AS1 functioned as an oncogene and autophagy promoter in hepatocellular carcinoma through sponging hsa-miR-30b-5p;
     (发表于Biochemical and Biophysical Research Communications,作者:Jiahong Dong)
   35、miR-186 inhibits cell proliferation in multiple myeloma by repressing Jagged1;
     (2016年发表于Biochemical and Biophysical Research Communications,作者:Jun Peng)
   36、House dust mite extract induces growth factor expression in nasal mucosa by activating the PI3K/Akt/HIF-1α pathway;
     (2016年发表于Biochem Biophys Res Commun,作者:Zhou H)
   37、Sitagliptin inhibits vascular inflammation via the SIRT6-dependent signaling pathway;
     (2019年发表于International Immunopharmacol,作者  Zhao F)
   38、Aldehyde dehydrogenase 2 activation in aged heart improves the autophagy by reducing the carbonyl modification on SIRT1;
     (2016年发表于Oncotarget,作者:Heng Ma)
   39、A novel role of microRNA 17-5p in the modulation of circadian rhythm;
     (2016年发表于Sci Rep,作者:Ji-Min Cao )
   40、Excess iodine promotes apoptosis of thyroid follicular epithelial cells by inducing autophagy suppression and is associated with Hashimoto thyroiditis disease;
     (2016年发表于J Autoimmun,作者:Xiao Y)
 
 
 
 
Chinese, Simplified
 
服务分类: