T细胞专用腺病毒

 

原代T细胞的基因操作利器

 

T细胞及其特性简介

 

TXIBAO(T cell、TLINBAXIBAO/T lymphocyte)SHIDEYIZHONG,ZAIZHONGBANYANZHEZHONGYAODEJIAOSE。TXIBAOZAIXIONGXIANNEIFENHUACHENGSHU,CHENGSHUHOUYIJUYUZHOUWEIZHONG。SUIZHEXIBAOMIANYIZHILIAODERIYISHENRU,TXIBAODEJIYINGONGCHENGHUAGAIZAOXIANDEYUELAIYUEZHONGYAO。

RANHOU,TXIBAODEJIYINCAOZUOQUEYICHANGKUNNAN,PUTONGDEDNAZHILIHENNANZHUANRAN,JISHISHIBINGDUJIEDAODEJIYINZHUANRANCAOZUO,YEYOUBUSHAONANTI,ZHUYAOTIXIANZAIJIGEFANGMIAN:

 

1.CHANGGUIDEMANBINGDU、NIZHUANLUBINGDU,YOUYU1)TXIBAOMOSHANGSHOUTIDEBUWENDING;2)FEIJIHUODETXIBAOBENSHENWUZENGZHINENGLI;3)TXIBAONEIBUYOUZUAIFANZHUANLUDEJIZHIDENGYUANYINERDAOZHIMANBINGDUHENIZHUANLUBINGDUDETXIBAOGANRANNENGLIPIANRUO,XUYAODUOCIZHONGFUGANRAN,QIEDUIYUSHIYANCAOZUODETIAOJIANYAOQIUSHIFENKEKE。

 

2.TIWAIPEIYANGDETXIBAOBENSHENSHIFENMINGANQIECUIRUO,MANBINGDUGANRANDUIXIBAODEZHUANGTAIYINGXIANGFEICHANGDA。

 

3.TXIBAOSHIXUANFUXIBAO,JINYIBUZENGDALEBINGDUYOUXIAODENANDU。

 

 

汉恒原代T细胞感染实例——使用悬浮细胞专用腺病毒ads感染

 

悬浮细胞专用腺病毒Ads感染小鼠原代CD4+ T细胞

 

 

XIBAO:XIAOSHUYUANDAICD4+ TXIBAO

CIJIFANGSHI:CD3/CD28KANGTIHEIL-2JIHUO48h

BINGDU:Ads-GFP, 1×10^10 PFU/ml

SHIYONGFANGSHI:MOI=500

 

 

悬浮细胞专用腺病毒Ads感染人原代CD4+ T细胞

 

 

XIBAO:RENYUANDAICD4+ TXIBAO

CIJIFANGSHI:CD3/CD28KANGTIHEIL-2JIHUO48h

BINGDU:Ads-GFP, 1×10^10 PFU/ml

SHIYONGFANGSHI:MOI=100

 

 

汉恒原代T细胞感染实例——使用慢病毒和逆转录病毒感染

 

原代T细胞专用病毒-慢病毒感染人原代CD4+ T细胞

 

 

XIBAO:RENYUANDAICD4+ TXIBAO

CIJIFANGSHI:CD3/CD28KANGTIHEIL-2JIHUO48h

BINGDU:hTLv-GFP,1*10^8 TU/ml

SHIYONGFANGSHI:MOI=50

 

原代T细胞专用病毒-逆转录病毒感染小鼠原代CD4+ T细胞

 

 

XIBAO:XIAOSHUYUANDAICD4+ TXIBAO

CIJIFANGSHI:CD3/CD28KANGTIHEIL-2JIHUO48 h

BINGDU:TXIBAOZHUANYONGNIZHUANLUBINGDUmTRv-GFP,1×108 TU/ml

SHIYONGFANGSHI:MOI=100

 

 

 参考文献

 

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