稳定细胞株筛选

 

亚博app官网简介

 

YANJIUMOUGEJIYINDEGONGNENGZUICHANGYONGDESHOUDUANSHIZAISUZHUXIBAOZHONGGUOLIANGBIAODAHUOZHETONGGUORNAGANRAODEFANGFAknock-downGAIJIYIN,CHANGGUISHOUDUANYOUSHUNSHIZHUANRANHESHAIXUANWENDINGXIBAOXI。

 

SHAIXUANCHUGAIJIYINDEGUOBIAODAHUOZHERNAGANRAODEWENDINGXIBAOXIHUIJININDESHIYANDAILAIJIDADEBIANLI。YOULEWENDINGXIBAOXI,HOUQIDECo-IPHUOZHEPull-DownSHIYANHUIFEICHANGBIANLI;WENDINGBIAODAZHONGZUYINGGUANGDANBAIDEXIBAOXIKEYIRANGNINDONGTAIDEGUANCHAFENZIZAIXIBAOZHONGDEYUNDONG。 GOUJIANWENDINGXIBAOXIDEJIBENYUANLISHIJIANGWAIYUANDNAKELONGDAOJUYOUMOUZHONGKANGXINGDEZAITISHANG,ZAITIBEIZHUANRANDAOSUZHUXIBAOBINGZHENGHEDAOSUZHURANSETIZHONG,YONGZAITIZHONGSUOHANDEKANGXINGBIAOZHIJINXINGSHAIXUAN。ZUICHANGYONGDEZHENHEBIAODAZAITIDEKANGXINGSHAIXUANBIAOZHIWUYOUXINMEISU(neomycin)、CHAOMEISU(hygromycin)HEPIAOLINGMEISU(puromycin),CHANGYONGG418LAIDAITIXINMEISUJINXINGXUANZEXINGSHAIXUAN,SHAIXUANDEDAOKEWENDINGBIAODAMUDEDANBAI,HUOZHEWENDINGBIAODACHENMOTEDINGJIYINDEXIBAOZHU。

 

 

筛选稳定细胞系的方法

 

1)ZHUANRANZHILIHOU,DANKELONGFANGFASHAIXUANWENDINGXIBAOXI

 

2)MANBINGDUGANRANSHAIXUANWENDINGXIBAOXI

 

MANBINGDUGANRANFANGFAJIAOZHILIZHUANRANSHAIXUANDANKELONGFANGFAGENGFANGBIANHEGAOXIAO,SHIMUQIANZHULIUDEWENDINGXIBAOXISHAIXUANFANGFA。MANBINGDUJIHUKEYIGANRANSUOYOUZHONGLEIDEXIBAO,BINGQIEZAIGANRANHOUKEYIZHENGHEDAOSHOUGANRANXIBAODEJIYINZU,JINXINGZHANGSHIJIANDEWENDINGBIAODA,YINCI,MANBINGDUCHANGYONGYUZHIBEIWENDINGBIAODA/CHENMOTEDINGJIYINDEDANKELONGXIBAOZHU。

 

HANHENGSHENGWUGOUJIANBAOZHUANGDEDAIpuromycinKANGXINGDEMANBINGDU,ZAIGANRANMUDEXIBAOHOU,KETONGGUOJIARUpuromycinJINXINGXUANZEXINGSHAIXUAN,CONGERHUODEWENDINGBIAODAshRNAHUOZHETEDINGJIYINDEXIBAOZHU。WOMENTIGONGBINGDUBAOZHUANGFUWU(NINKEYIZIJIWANCHENGWENTAIXIBAOSHAIXUAN),HUOZHEYIZHANSHIDEWENDINGXIBAOXIDESHAIXUANFUWU。

 

 

服务流程

 

 

 

我们的优势

 

● 种子优良--  选低代数细胞来源,无支原体无黑胶虫;

HANHENGSHENGWUSUOYOUDEXIBAOJUNCONGZHONGKEYUANXIBAOKUGOUMAI,DAISHUDIWUWURAN,KETIGONGSTRJIANCEBAOGAO

● 性状稳定-- 严 酷的筛选及靶基因表达验证体系;

HANHENGSHENGWUSUOYOUDEWENZHUANZHUDOUCAIYONGRT-qPCRYANZHENG,BAOZHENGBIAODASHUIPING

● 活力无损-- 严 格的建系后连续3代细胞增殖活力评价

 

 

 

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