亚博app官网包装和保存条件

 

 

 

SHIJIHEYU-20℃BAOCUN。Lysis Buffer -20℃KEBAOCUNYINIAN。FANYINGSHIJI（Luciferase Reaction ReagentHELuciferase Reaction Reagent II）JIARUDIWUHOU，FENZHUANG、BIGUANGBAOCUN；-70℃KEBAOCUNYINIAN，-20℃KEBAOCUNYIGEYUE；BIMIANFANFUDONGRONG。SHIJISHIYONGQIANXUPINGHENGZHISHIWEN。

 

 

实验原理

 

  YINGHUOCHONGYINGGUANGSUMEI（firefly luciferase, fluc）HEHAISHENYINGGUANGSUMEI（renilla luciferase, rluc）KEFENBIECUIHUAYINGGUANGSU（luciferin）HEQIANGCHANGSU（coelenterazine）CHANSHENGSHENGWUYINGGUANGFANYING，FANYINGGUOCHENGRUXIATUSUOSHI。

 

 

 

 

ZAISHUANGYINGGUANGSUMEIFANYINGZHONG，SHOUXIANYONGLuciferase Reaction ReagentJIANCEfLucXINHAO；SUIHOU，ZHIJIEJIARULuciferase Reaction Reagent II，JIANGfLucDEXINHAOCUMIE，TONGSHICHANSHENGrLucXINHAO。GAIFANGFAJUYOUCAOZUOJIANDAN、JIANCEKUAIJIE、LINGMINDUGAODENGYOUDIAN。

 

 

实验步骤

 

1.PEIZHISHIJI

 

（1）Lysis Buffer：

Lysis Buffer (5X) YONGSHUIXISHI5BEI，PEIZHICHENGGONGZUOYE；

 

（2）Luciferase Reaction Reagent：

YONGLuciferase Reaction Buffer（10 ml）RONGJIELuciferase Reaction SubstrateGANFEN（DUANZANLIXINZAIKAIGAI），HUNYUNHOUFENZHUANG、BIGUANGBAOCUN，BIMIANFANFUDONGRONG；

 

（3）Luciferase Reaction Reagent II：

YONGLuciferase Reaction Buffer II（10 ml）XISHI、HUNHELuciferase Reaction Substrate II（DUANZANLIXINZAIKAIGAI），FENZHUANG、BIGUANGBAOCUN，BIMIANFANFUDONGRONG。

 

2.LIEJIEXIBAO

 

XIBAOLIEJIESHIMIDUYINGXIAOYU95%，XIBAOSHENGZHANGGUOMI，YIDAOZHILIEJIEBUCHONGFEN；ZHILISHUNZHUAN、BINGDUGANRANXIBAOHOU，XUPEIYANGYIDUANSHIJIAN（YIBAN48XIAOSHI）SHIYINGGUANGSUMEICHONGFENBIAODA；DIAOJIESHIYANCANSHU，YIMANZUSHANGSHUTIAOJIAN；

 

QUCHUPEIYANGJI，YONGPBSRUNXIYIBIAN，ZHUYIBUYAOJIANGXIBAOCHUIQI；JIARUSHILIANGDE1X Lysis Buffer，TUIJIANYONGLIANGJIANXIABIAO；

 

 

 

JIANGXIBAOPEIYANGBANZHIYUYAOCHUANGSHANG，SHIWEN、QINGHUANGFUYU15FENZHONG；

 

JIANGLIEJIEWUZHUANYIDAOXINDEEPGUANZHONG，4℃、12,000rpmLIXIN1FENZHONG，QUSHANGQINGBEIYONG。

 

3.YINGGUANGJIANCE

 

QU20μlLIEJIEWU，JIARU100μlPINGHENGZHISHIWENDELuciferase Reaction Reagent，HUNYUNHOUYUMEIBIAOYIZHONGJIANCEfLucXINHAO；

 

RANHOUJIARU100μlPINGHENGZHISHIWENDELuciferase Reaction Reagent II，HUNYUNHOUYUMEIBIAOYIZHONGJIANCErLucXINHAO。