双荧光素酶检测试剂盒

 

 

亚博app官网包装和保存条件

 

 

 

SHIJIHEYU-20℃BAOCUN。Lysis Buffer -20℃KEBAOCUNYINIAN。FANYINGSHIJI(Luciferase Reaction ReagentHELuciferase Reaction Reagent II)JIARUDIWUHOU,FENZHUANG、BIGUANGBAOCUN;-70℃KEBAOCUNYINIAN,-20℃KEBAOCUNYIGEYUE;BIMIANFANFUDONGRONG。SHIJISHIYONGQIANXUPINGHENGZHISHIWEN。

 

 

实验原理

 

  YINGHUOCHONGYINGGUANGSUMEI(firefly luciferase, fluc)HEHAISHENYINGGUANGSUMEI(renilla luciferase, rluc)KEFENBIECUIHUAYINGGUANGSU(luciferin)HEQIANGCHANGSU(coelenterazine)CHANSHENGSHENGWUYINGGUANGFANYING,FANYINGGUOCHENGRUXIATUSUOSHI。

 

 

 

 

ZAISHUANGYINGGUANGSUMEIFANYINGZHONG,SHOUXIANYONGLuciferase Reaction ReagentJIANCEfLucXINHAO;SUIHOU,ZHIJIEJIARULuciferase Reaction Reagent II,JIANGfLucDEXINHAOCUMIE,TONGSHICHANSHENGrLucXINHAO。GAIFANGFAJUYOUCAOZUOJIANDAN、JIANCEKUAIJIE、LINGMINDUGAODENGYOUDIAN。

 

 

实验步骤

 

1.PEIZHISHIJI

 

(1)Lysis Buffer:

Lysis Buffer (5X) YONGSHUIXISHI5BEI,PEIZHICHENGGONGZUOYE;

 

(2)Luciferase Reaction Reagent:

YONGLuciferase Reaction Buffer(10 ml)RONGJIELuciferase Reaction SubstrateGANFEN(DUANZANLIXINZAIKAIGAI),HUNYUNHOUFENZHUANG、BIGUANGBAOCUN,BIMIANFANFUDONGRONG;

 

(3)Luciferase Reaction Reagent II:

YONGLuciferase Reaction Buffer II(10 ml)XISHI、HUNHELuciferase Reaction Substrate II(DUANZANLIXINZAIKAIGAI),FENZHUANG、BIGUANGBAOCUN,BIMIANFANFUDONGRONG。

 

2.LIEJIEXIBAO

 

XIBAOLIEJIESHIMIDUYINGXIAOYU95%,XIBAOSHENGZHANGGUOMI,YIDAOZHILIEJIEBUCHONGFEN;ZHILISHUNZHUAN、BINGDUGANRANXIBAOHOU,XUPEIYANGYIDUANSHIJIAN(YIBAN48XIAOSHI)SHIYINGGUANGSUMEICHONGFENBIAODA;DIAOJIESHIYANCANSHU,YIMANZUSHANGSHUTIAOJIAN;

 

QUCHUPEIYANGJI,YONGPBSRUNXIYIBIAN,ZHUYIBUYAOJIANGXIBAOCHUIQI;JIARUSHILIANGDE1X Lysis Buffer,TUIJIANYONGLIANGJIANXIABIAO;

 

 

 

JIANGXIBAOPEIYANGBANZHIYUYAOCHUANGSHANG,SHIWEN、QINGHUANGFUYU15FENZHONG;

 

JIANGLIEJIEWUZHUANYIDAOXINDEEPGUANZHONG,4℃、12,000rpmLIXIN1FENZHONG,QUSHANGQINGBEIYONG。

 

3.YINGGUANGJIANCE

 

QU20μlLIEJIEWU,JIARU100μlPINGHENGZHISHIWENDELuciferase Reaction Reagent,HUNYUNHOUYUMEIBIAOYIZHONGJIANCEfLucXINHAO;

 

RANHOUJIARU100μlPINGHENGZHISHIWENDELuciferase Reaction Reagent II,HUNYUNHOUYUMEIBIAOYIZHONGJIANCErLucXINHAO。

 

Chinese, Simplified
 
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