亚博app官网包装和保存条件

 

 

 

 

SHIJIHEYU-20℃BAOCUN。Lysis Buffer -20℃KEBAOCUNYINIAN。FANYINGSHIJI（Luciferase Reaction Reagent）JIARUDIWUHOU，FENZHUANG、BIGUANGBAOCUN；-70℃KEBAOCUNYINIAN，-20℃KEBAOCUNYIGEYUE；BIMIANFANFUDONGRONG。SHIJISHIYONGQIANXUPINGHENGZHISHIWEN。

 

 

 

实验原理

 

 

YINGHUOCHONGYINGGUANGSUMEI（firefly luciferase, fluc）NENGCUIHUAYINGGUANGSU（luciferin）CHANSHENGSHENGWUYINGGUANGFANYING，FANYINGGUOCHENGRUXIATUSUOSHI。

 

 

 

 

BENJIANCESHIJIHEYIYINGGUANGSUWEIDIWU，JIANCEYINGGUANGSUMEI（firefly）BAOGAOJIYINDEHUOXING。GAIFANGFAJUYOUCAOZUOJIANDAN、JIANCEKUAIJIE、LINGMINDUGAODENGYOUDIAN。

 

 

实验步骤

 

1.PEIZHISHIJI

 

（1）Lysis Buffer：

Lysis Buffer (5X) YONGSHUIXISHI5BEI，PEIZHICHENGGONGZUOYE；

 

（2）Luciferase Reaction Reagent：

YONGLuciferase Reaction Buffer（10 ml）RONGJIELuciferase Reaction SubstrateGANFEN（DUANZANLIXINZAIKAIGAI），HUNYUNHOUFENZHUANG、BIGUANGBAOCUN，BIMIANFANFUDONGRONG。

 

 

2.LIEJIEXIBAO

 

XIBAOLIEJIESHIMIDUYINGXIAOYU95%，XIBAOSHENGZHANGGUOMI，YIDAOZHILIEJIEBUCHONGFEN；ZHILISHUNZHUAN、BINGDUGANRANXIBAOHOU，XUPEIYANGYIDUANSHIJIAN（YIBAN48XIAOSHI）SHIYINGGUANGSUMEICHONGFENBIAODA；DIAOJIESHIYANCANSHU，YIMANZUSHANGSHUTIAOJIAN；

 

QUCHUPEIYANGJI，YONGPBSRUNXIYIBIAN，ZHUYIBUYAOJIANGXIBAOCHUIQI；JIARUSHILIANGDELysis Buffer，TUIJIANYONGLIANGJIANXIABIAO；

 

 

 

JIANGXIBAOPEIYANGBANZHIYUYAOCHUANGSHANG，SHIWEN、QINGHUANGFUYU15FENZHONG；

JIANGLIEJIEWUZHUANYIDAOXINDEEPGUANZHONG，4℃、12,000rpmLIXIN1FENZHONG，QUSHANGQINGBEIYONG。

 

 

3.YINGGUANGJIANCE

 

QU20μlLIEJIEWU，JIARU100μlPINGHENGZHISHIWENDELuciferase Reaction Reagent，HUNYUNHOUYUMEIBIAOYIZHONGJIANCEfLucXINHAO。